# Series 1B- Two stressor effects on Tp-1335-follow-up experiments # Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (mu) # of Thalassiosira pseudonana CCMP 1335 conducted to supplement series 1A experiments. # PI: U. Passow (UCSB) # version: 2018-09-04 # ========================= expt temp light_level date_expt ------------------------- 3 22 high 2017-06-26 ========================= light day avg_OD680 avg_OD720 F0 cell_counts QY Chla_repl_1 Chla_repl_2 POC_repl_1 POC_repl_2 PON_repl_1 PON_repl_2 ------------------------- 140 0 nd nd nd 100015 nd nd nd nd nd nd nd 140 1 0.053 0.033 2090 606000 0.75 nd nd nd nd nd nd 140 2 0.115 0.059 5541 978333 0.74 227.1 nd 20189 nd 2056 nd 140 3 0.167 0.087 7486 920000 0.71 194.0 nd 22030 nd 2597 nd 140 4 0.197 0.110 8567 958333 0.7 287.7 413.0 27942 27848 3835 3568 170 0 nd nd nd 100015 nd nd nd nd nd nd nd 170 1 0.051 0.019 2044 563000 0.75 nd nd nd nd nd nd 170 2 0.088 0.039 5509 1073333 0.74 152.0 nd 21598 nd 2110 nd 170 3 0.173 0.083 7875 945000 0.72 189.1 nd 27607 nd 3516 nd 170 4 0.214 0.113 9149 806667 0.71 291.3 319.7 35834 33833 5645 4367 200 0 nd nd nd 100015 nd nd nd nd nd nd nd 200 1 0.037 0.000 1910 617000 0.74 nd nd nd nd nd nd 200 2 0.117 0.035 5551 1116667 0.74 166.8 nd 20846 nd 1407 nd 200 3 0.176 0.069 7485 911667 0.7 278.5 nd 25711 nd 3030 nd 200 4 0.213 0.104 8421 880000 0.7 248.6 248.8 31850 29490 4846 3781 230 0 nd nd nd 100015 nd nd nd nd nd nd nd 230 1 0.060 0.039 1909 532000 0.75 nd nd nd nd nd nd 230 2 0.127 0.067 5471 1116667 0.74 250.6 nd 28509 nd 2651 nd 230 3 0.198 0.101 8283 1038333 0.72 290.1 nd 26049 nd 2921 nd 230 4 0.245 0.136 9237 1018333 0.71 322.3 153.1 29906 31756 4048 3515 260 0 nd nd nd 100015 nd nd nd nd nd nd nd 260 1 0.054 0.036 1868 501000 0.75 nd nd nd nd nd nd 260 2 0.121 0.073 5251 1185000 0.74 291.2 nd 18367 nd 1785 nd 260 3 0.171 0.112 7620 855000 0.71 214.3 nd 23570 nd 2326 nd 260 4 0.193 0.119 8992 1005000 0.69 121.5 207.0 28244 26791 3568 3302 290 0 nd nd nd 100015 nd nd nd nd nd nd nd 290 1 0.036 0.017 1814 521000 0.76 nd nd nd nd nd nd 290 2 0.102 0.052 5145 1031667 0.74 208.8 nd 18799 nd 1785 nd 290 3 0.155 0.084 7316 886667 0.7 338.2 nd 22255 nd 2597 nd 290 4 0.178 0.106 8382 1003333 0.68 385.7 239.0 24582 24336 3195 3036 320 0 nd nd nd 100015 nd nd nd nd nd nd nd 320 1 0.041 0.029 1885 461000 0.75 nd nd nd nd nd nd 320 2 0.100 0.054 4884 1056667 0.75 115.8 nd 15851 nd 1515 nd 320 3 0.170 0.096 7182 953333 0.72 320.8 nd 21053 nd 2651 nd 320 4 0.209 0.131 8338 906667 0.69 229.9 215.3 28584 27772 4207 3568 350 0 nd nd nd 100015 nd nd nd nd nd nd nd 350 1 0.079 0.027 1794 484000 0.75 nd nd nd nd nd nd 350 2 0.100 0.045 4788 948333 0.75 136.1 nd 16226 nd 1515 nd 350 3 0.163 0.070 7673 1108333 0.72 220.5 nd 21354 nd 2434 nd 350 4 0.197 0.092 9231 935000 0.71 467.2 186.8 26847 25507 3409 3249 ========================= expt temp light_level date_expt ------------------------- 4 22 low 2017-07-07 ========================= light day avg_OD680 avg_OD720 F0 cell_counts QY Chla_repl_1 Chla_repl_2 POC_repl_1 POC_repl_2 PON_repl_1 PON_repl_2 ------------------------- 35 0 nd nd nd 100006 nd nd nd nd nd nd nd 35 3 0.043 0.031 1092 221000 0.74 nd nd nd nd nd nd 35 4 0.190 0.162 1538 254000 0.74 nd nd nd nd nd nd 35 5 0.348 0.318 1832 340000 0.74 nd nd nd nd nd nd 35 6 0.441 0.405 2967 508333 0.73 110.7 nd 86788 nd 10592 nd 35 7 0.528 0.494 3328 500000 0.72 130.8 nd 101764 nd 10750 nd 35 8 0.554 0.514 3770 446667 0.67 117.0 57.5 107660 106532 10844 11003 50 0 nd nd nd 100006 nd nd nd nd nd nd nd 50 3 0.059 0.046 1158 206000 0.74 nd nd nd nd nd nd 50 4 0.167 0.145 1569 263000 0.72 nd nd nd nd nd nd 50 5 0.328 0.292 2020 406000 0.73 nd nd nd nd nd nd 50 6 0.402 0.385 3124 600000 0.73 289.1 nd 85639 nd 10486 nd 50 7 0.472 0.463 3872 550000 0.71 160.3 nd 102047 nd 10697 nd 50 8 0.569 0.539 3104 488333 0.67 86.8 126.5 112585 115593 10632 10527 65 0 nd nd nd 100006 nd nd nd nd nd nd nd 65 3 0.065 0.052 1261 245000 0.73 nd nd nd nd nd nd 65 4 0.178 0.154 1839 336000 0.74 nd nd nd nd nd nd 65 5 0.355 0.315 2201 458000 0.75 nd nd nd nd nd nd 65 6 0.469 0.421 3065 608333 0.73 326.3 nd 83548 nd 10223 nd 65 7 0.541 0.500 3888 566667 0.7 71.7 nd 98185 nd 10486 nd 65 8 0.567 0.542 2822 425000 0.67 98.3 142.2 111194 109183 11108 10579 80 0 nd nd nd 100006 nd nd nd nd nd nd nd 80 3 0.068 0.048 1102 201111 0.76 nd nd nd nd nd nd 80 4 0.183 0.144 1442 254000 0.74 nd nd nd nd nd nd 80 5 0.346 0.288 2149 421000 0.75 nd nd nd nd nd nd 80 6 0.452 0.390 2681 516667 0.73 264.6 nd 83020 nd 10697 nd 80 7 0.527 0.463 3088 738333 0.7 101.1 nd 96527 nd 10539 nd 80 8 0.601 0.521 3884 776667 0.69 142.7 128.1 111570 107754 11108 10579 95 0 nd nd nd 100006 nd nd nd nd nd nd nd 95 3 0.068 0.054 1216 216000 0.75 nd nd nd nd nd nd 95 4 0.158 0.132 1931 347000 0.73 nd nd nd nd nd nd 95 5 0.340 0.306 2344 489000 0.75 nd nd nd nd nd nd 95 6 0.446 0.411 3150 506667 0.74 219.2 nd 80609 nd 10434 nd 95 7 0.522 0.485 3008 596667 0.71 91.4 nd 93777 nd 10223 nd 95 8 0.566 0.524 3153 628333 0.67 78.1 68.1 98298 97753 10632 10315 110 0 nd nd nd 100006 nd nd nd nd nd nd nd 110 3 0.048 0.035 1453 247000 0.73 nd nd nd nd nd nd 110 4 0.220 0.185 1900 331000 0.73 nd nd nd nd nd nd 110 5 0.407 0.352 2240 453000 0.74 nd nd nd nd nd nd 110 6 0.536 0.469 3613 586667 0.76 276.2 nd 87052 nd 9854 nd 110 7 0.613 0.549 3824 661667 0.69 164.2 nd 102894 nd 10328 nd 110 8 0.650 0.591 4092 570000 0.66 95.7 118.3 114916 113657 10738 10738 125 0 nd nd nd 100006 nd nd nd nd nd nd nd 125 3 0.055 0.040 1233 234000 0.74 nd nd nd nd nd nd 125 4 0.169 0.144 1568 299000 0.74 nd nd nd nd nd nd 125 5 0.313 0.280 2580 439000 0.75 nd nd nd nd nd nd 125 6 0.436 0.403 2789 451667 0.74 222.3 nd 83416 nd 10750 nd 125 7 0.514 0.484 3252 563333 0.72 38.4 nd 102744 nd 10065 nd 125 8 0.571 0.544 3044 611667 0.68 122.1 175.9 109164 110837 10632 10791 140 0 nd nd nd 100006 nd nd nd nd nd nd nd 140 3 0.100 0.080 1193 210556 0.74 nd nd nd nd nd nd 140 4 0.218 0.179 1557 351000 0.73 nd nd nd nd nd nd 140 5 0.390 0.326 1751 328000 0.75 nd nd nd nd nd nd 140 6 0.469 0.413 2419 403333 0.76 159.3 nd 82719 nd 10012 nd 140 7 0.574 0.502 2784 515000 0.71 34.5 nd 98599 nd 10645 nd 140 8 0.618 0.554 2360 408333 0.69 91.9 114.9 112924 108938 10474 10051